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leptin neutralizing antibody lep ab  (Fisher Scientific)

 
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    Fisher Scientific leptin neutralizing antibody lep ab
    ( A ) Experimental schematic showing the collection of breast adipose tissue conditioned media (CM) from lean and overweight/obese women. ( B ) MCF-10A cells were treated with CM for 24 hours. DNA damage assessed by IF (#γH2AX foci/100 cells) is shown correlated with BMI in BRCA1 +/- (n=36 CM cases) and ( C ) BRCA2 +/- (n=13 CM cases) MCF-10A cells. Spearman’s rank correlation coefficient (ρ) and associated P value are shown along with 95% confidence intervals. ( D ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( E ) primary BRCA1 +/- breast epithelial cells treated with <t>leptin</t> (400ng/µl) for 24 hours. ( F ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, obese (ob) CM, or ob CM in the presence of a leptin <t>neutralizing</t> antibody (Lep Ab). ( G ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( H ) primary BRCA2 +/- breast epithelial cells treated with insulin (100nM) for 24 hours. ( I ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, ob CM, or ob CM in the presence of PI3K inhibitor BKM120 (1µM). Student’s t-test was used to determine significant differences in ( D-I) . All experiments in MCF-10A cells were conducted a minimum of two times with representative results from one experiment shown. Data in primary cells were generated from cells treated in triplicate. Data is presented as mean +/- SD. * P <0.05, ** P <0.01, *** P <0.001, ns= not significant.
    Leptin Neutralizing Antibody Lep Ab, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptin neutralizing antibody lep ab/product/Fisher Scientific
    Average 90 stars, based on 1 article reviews
    leptin neutralizing antibody lep ab - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Obesity promotes breast epithelium DNA damage in BRCA mutation carriers"

    Article Title: Obesity promotes breast epithelium DNA damage in BRCA mutation carriers

    Journal: bioRxiv

    doi: 10.1101/2022.07.29.502090

    ( A ) Experimental schematic showing the collection of breast adipose tissue conditioned media (CM) from lean and overweight/obese women. ( B ) MCF-10A cells were treated with CM for 24 hours. DNA damage assessed by IF (#γH2AX foci/100 cells) is shown correlated with BMI in BRCA1 +/- (n=36 CM cases) and ( C ) BRCA2 +/- (n=13 CM cases) MCF-10A cells. Spearman’s rank correlation coefficient (ρ) and associated P value are shown along with 95% confidence intervals. ( D ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( E ) primary BRCA1 +/- breast epithelial cells treated with leptin (400ng/µl) for 24 hours. ( F ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, obese (ob) CM, or ob CM in the presence of a leptin neutralizing antibody (Lep Ab). ( G ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( H ) primary BRCA2 +/- breast epithelial cells treated with insulin (100nM) for 24 hours. ( I ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, ob CM, or ob CM in the presence of PI3K inhibitor BKM120 (1µM). Student’s t-test was used to determine significant differences in ( D-I) . All experiments in MCF-10A cells were conducted a minimum of two times with representative results from one experiment shown. Data in primary cells were generated from cells treated in triplicate. Data is presented as mean +/- SD. * P <0.05, ** P <0.01, *** P <0.001, ns= not significant.
    Figure Legend Snippet: ( A ) Experimental schematic showing the collection of breast adipose tissue conditioned media (CM) from lean and overweight/obese women. ( B ) MCF-10A cells were treated with CM for 24 hours. DNA damage assessed by IF (#γH2AX foci/100 cells) is shown correlated with BMI in BRCA1 +/- (n=36 CM cases) and ( C ) BRCA2 +/- (n=13 CM cases) MCF-10A cells. Spearman’s rank correlation coefficient (ρ) and associated P value are shown along with 95% confidence intervals. ( D ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( E ) primary BRCA1 +/- breast epithelial cells treated with leptin (400ng/µl) for 24 hours. ( F ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, obese (ob) CM, or ob CM in the presence of a leptin neutralizing antibody (Lep Ab). ( G ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( H ) primary BRCA2 +/- breast epithelial cells treated with insulin (100nM) for 24 hours. ( I ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, ob CM, or ob CM in the presence of PI3K inhibitor BKM120 (1µM). Student’s t-test was used to determine significant differences in ( D-I) . All experiments in MCF-10A cells were conducted a minimum of two times with representative results from one experiment shown. Data in primary cells were generated from cells treated in triplicate. Data is presented as mean +/- SD. * P <0.05, ** P <0.01, *** P <0.001, ns= not significant.

    Techniques Used: Generated



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    leptin neutralizing antibody lep ab  (Fisher Scientific)
    90
    Fisher Scientific leptin neutralizing antibody lep ab
    ( A ) Experimental schematic showing the collection of breast adipose tissue conditioned media (CM) from lean and overweight/obese women. ( B ) MCF-10A cells were treated with CM for 24 hours. DNA damage assessed by IF (#γH2AX foci/100 cells) is shown correlated with BMI in BRCA1 +/- (n=36 CM cases) and ( C ) BRCA2 +/- (n=13 CM cases) MCF-10A cells. Spearman’s rank correlation coefficient (ρ) and associated P value are shown along with 95% confidence intervals. ( D ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( E ) primary BRCA1 +/- breast epithelial cells treated with <t>leptin</t> (400ng/µl) for 24 hours. ( F ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, obese (ob) CM, or ob CM in the presence of a leptin <t>neutralizing</t> antibody (Lep Ab). ( G ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( H ) primary BRCA2 +/- breast epithelial cells treated with insulin (100nM) for 24 hours. ( I ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, ob CM, or ob CM in the presence of PI3K inhibitor BKM120 (1µM). Student’s t-test was used to determine significant differences in ( D-I) . All experiments in MCF-10A cells were conducted a minimum of two times with representative results from one experiment shown. Data in primary cells were generated from cells treated in triplicate. Data is presented as mean +/- SD. * P <0.05, ** P <0.01, *** P <0.001, ns= not significant.
    Leptin Neutralizing Antibody Lep Ab, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptin neutralizing antibody lep ab/product/Fisher Scientific
    Average 90 stars, based on 1 article reviews
    leptin neutralizing antibody lep ab - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Experimental schematic showing the collection of breast adipose tissue conditioned media (CM) from lean and overweight/obese women. ( B ) MCF-10A cells were treated with CM for 24 hours. DNA damage assessed by IF (#γH2AX foci/100 cells) is shown correlated with BMI in BRCA1 +/- (n=36 CM cases) and ( C ) BRCA2 +/- (n=13 CM cases) MCF-10A cells. Spearman’s rank correlation coefficient (ρ) and associated P value are shown along with 95% confidence intervals. ( D ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( E ) primary BRCA1 +/- breast epithelial cells treated with leptin (400ng/µl) for 24 hours. ( F ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, obese (ob) CM, or ob CM in the presence of a leptin neutralizing antibody (Lep Ab). ( G ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( H ) primary BRCA2 +/- breast epithelial cells treated with insulin (100nM) for 24 hours. ( I ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, ob CM, or ob CM in the presence of PI3K inhibitor BKM120 (1µM). Student’s t-test was used to determine significant differences in ( D-I) . All experiments in MCF-10A cells were conducted a minimum of two times with representative results from one experiment shown. Data in primary cells were generated from cells treated in triplicate. Data is presented as mean +/- SD. * P <0.05, ** P <0.01, *** P <0.001, ns= not significant.

    Journal: bioRxiv

    Article Title: Obesity promotes breast epithelium DNA damage in BRCA mutation carriers

    doi: 10.1101/2022.07.29.502090

    Figure Lengend Snippet: ( A ) Experimental schematic showing the collection of breast adipose tissue conditioned media (CM) from lean and overweight/obese women. ( B ) MCF-10A cells were treated with CM for 24 hours. DNA damage assessed by IF (#γH2AX foci/100 cells) is shown correlated with BMI in BRCA1 +/- (n=36 CM cases) and ( C ) BRCA2 +/- (n=13 CM cases) MCF-10A cells. Spearman’s rank correlation coefficient (ρ) and associated P value are shown along with 95% confidence intervals. ( D ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( E ) primary BRCA1 +/- breast epithelial cells treated with leptin (400ng/µl) for 24 hours. ( F ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, obese (ob) CM, or ob CM in the presence of a leptin neutralizing antibody (Lep Ab). ( G ) DNA damage in BRCA1 +/- and BRCA2 +/- MCF-10A cells and in ( H ) primary BRCA2 +/- breast epithelial cells treated with insulin (100nM) for 24 hours. ( I ) DNA damage in BRCA1 +/- MCF-10A cells after 24-hour treatment with lean CM, ob CM, or ob CM in the presence of PI3K inhibitor BKM120 (1µM). Student’s t-test was used to determine significant differences in ( D-I) . All experiments in MCF-10A cells were conducted a minimum of two times with representative results from one experiment shown. Data in primary cells were generated from cells treated in triplicate. Data is presented as mean +/- SD. * P <0.05, ** P <0.01, *** P <0.001, ns= not significant.

    Article Snippet: In leptin neutralization studies, obese CM was pre-incubated with a leptin neutralizing antibody (Lep ab, 13.3μg/mL, Fisher Scientific #AF398) for 1 hour at 4°C and then cells were treated with lean or obese CM alone or obese CM + Lep ab.

    Techniques: Generated